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Microscopy for Biological Sciences (BIOL90001)

Graduate courseworkPoints: 12.5On Campus (Parkville)

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Overview

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Semester 1 - On Campus
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Microscopy is the key technique for imaging fine structure in biological specimens. This subject will introduce the range of methods and capabilities of light microscopy, scanning and transmission electron microscopy, and laser scanning confocal microscopy, as well as the methods of specimen preparation for standard histochemical and immunocytochemical techniques. The principles and scientific basis underpinning the various methods and techniques will be explained, and applications to current cutting-edge science and technology will be discussed. Practical and project work will include demonstration of equipment and analysis of images and data.

Intended learning outcomes

The objectives of this subject are to provide students with:

  • Understand the theoretical basis of optical and electron microscopy, and variations of these techniques.
  • Recognise how biological samples are prepared and imaged for different types of microscopies.
  • Critically evaluate microscopy images for quality and meaning.
  • Analyse microscopy images using image analysis techniques.
  • Apply this theoretical and practical knowledge to make informed choices for imaging technology and analysis of different samples.

Generic skills

On completion of this subject students should have developed the following generic skills:

  • Communication: The ability to communicate effectively in writing and orally
  • Critical thinking: The ability to think independently and critically
  • Analysis: The ability to analyse information and data
  • Information management: The ability to find, evaluate, and synthesize information

Last updated: 6 November 2025